Workshop: RNA Sequencing
On 8 and 9 October, NeSI and Genomics Aotearoa will be delivering an online workshop on RNA Sequencing. The workshop will be taught over two mornings from 9:00am - 1:00pm NZT on both days.
This workshop focuses on an RNA-seq data analysis workflow with the aim to identify differentially expressed genes.
On Day 1: We will cover quality control and read cleaning, alignment and quantification methods, and carry out an exploratory analysis of an example data set.
On Day 2: We will work through two methods which take unique statistical approaches to identifying differentially expressed genes. We will then look at over-representation analysis, a popular method to derive biological meaning from a list of differentially expressed genes.
Who this workshop is for?
This workshop is for New Zealand based researchers interested in RNA-seq data analysis. We assume attendees to be familiar with the basics of R ( e.g., you can copy basic functions like head, tail, or colSums, you are aware of how R stores files as Objects) and bash (e.g., can change directory with cd command, make a new folder with mkdir command, view contents using less command).
If you would like a refresher on R you can find one here. If you would like a refresher on bash you can find one here.
Setup
This is a fully online, hands-on workshop. This workshop material will delivered on the NeSI High Performance Computing (HPC) platform - there is no need to install any software for this workshop. Instructions on how to access the platform will be sent out with the confirmation letter to registrants.
Participants must have their own laptops and plan to participate actively. You will require a working web browser.
If you have any questions about these workshops, including whether they are suitable for you, please contact training@nesi.org.nz
